Microbial Biotechnology – Molecular Bacteriology – Virology

Brief Description

The laboratory of Microbial Biotechnology – Molecular Bacteriology – Virology of the Department of Biochemistry & Biotechnology was foundedin February 2004. The laboratory is implicated in teaching, undergraduate and postgraduate students as well asconducting basic and applied research in the field of Molecular Bacteriology and Virology. Director of the lab is Dr Dimitris Mossialos, Associate Professor in Microbial Biotechnology.

Research Interests

Bacteriology: Study of Greek bee products (corresponding bioactive molecules as well) microbiome. Study of honeybee (Apis melifera) microbiome and pathogens. Antibacterial properties and mode of action of Greek bee products. Microbial diversity in cultural heritage objects and biodeterioration.
Molecular microbiology with emphasis on Pseudomonad biology (High-affinity iron uptake systems, oxidative stress, cyanogenesis, gene regulation, antibiotic resistance) Biotechnological Application of Pseudomonads in Agriculture and Environment.
Virology: Molecular detection and identification of Enteroviruses from clinical and environmental samples, detection of recombination events and effects on virus circulation. Molecular detection and identification of HPVs and human gene polymorphism association with the development of cervical cancer. Development of molecular methods for the detection of replication activity in Enteroviruses. Molecular testing of enterovirus inactivation techniques.
Study of the anti-viral activity of Greek honeys and other beehive products. Molecular study of viral bee pathogens.


  1. Teaching & Research Staff
  2. Laboratory Teaching Staff
  3. PhD Students
    • Nikos Asoutis-Didaras

Research Projects-Funding

An indicative list of research projects funded during the period 2016-2022

  1. Project No 4954: “Antibacterial activity and mechanisms of action of honeys produced in Mt Olympus area against pathogenic bacteria”.Funding: Thessaly Prefecture, Greece. Duration: 13/07/2016 – 12/07/2018
  2. Project No 6101: “National Research Consortium “Roads of honeybee”. Funding: General Secretariat of Research and Technology, Greece. Duration: 15/05/2019 – 14/05/2021
  3. 2nd Call of PhD scholarships (ELIDEK).MsTsadila ChristinaPhD title: “Characterization of microbiome bioactivity and antimicrobial mechanisms of Greek honeys”. Duration 9/2019 –8/2022
  4. Project No 6738: Beebread and young adult bee microbiota as biomarker in Nosemosis “Study of the effectiveness of new – alternative methods”. Funding: Hellenic Ministry of Rural Development and Food: 26/02/2021 – 31/07/2022


15 indicative recent publications covering the period 2018-2022 are listed

  1. Kontogiannis, T., Dimitriou, T.G., Didaras, N.A., Mossialos, D. Antiviral activity of bee products (2022) Current Pharmaceutical Design, 28 (35), pp. 2867-2878 http://doi.org/10.2174/1381612828666220928110103
  2. Tsavea, E., Vardaka, F.-P., Savvidaki, E., Kellil, A., Kanelis, D., Bucekova, M., Grigorakis, S., Godocikova, J., Gotsiou, P., Dimou, M., Loupassaki, S., Remoundou, I., Tsadila, C., Dimitriou, T.G., Majtan, J., Tananaki, C., Alissandrakis, E., Mossialos, D. Physicochemical Characterization and Biological Properties of Pine Honey Produced across Greece (2022) Foods, 11 (7), 943, https://doi.org/10.3390/foods11070943
  3. Didaras, N.A., Dimitriou, T.G., Daskou, M., Karatasou, K., Mossialos, D. In Vitro Assessment of the Antiviral Activity of Greek Bee Bread And Bee Collected Pollen Against Enterovirus D68 (2022) Journal of Microbiology, Biotechnology and Food Sciences, 11 (4), e4859, https://doi.org/10.55251/jmbfs.4859
  4. Tsadila, C., Nikolaidis, M., Dimitriou, T.G., Kafantaris, I., Amoutzias, G.D., Pournaras, S., Mossialos, D. Antibacterial activity and characterization of bacteria isolated from diverse types of greek honey against nosocomial and foodborne pathogens (2021) Applied Sciences (Switzerland), 11 (13), 5801, https://doi.org/10.3390/app11135801
  5. Didaras, N.A., Kafantaris, I., Dimitriou, T.G., Mitsagga, C., Karatasou, K., Giavasis, I., Stagos, D., Amoutzias, G.D., Hatjina, F., Mossialos, D. Biological properties of bee bread collected from apiaries located across Greece (2021) Antibiotics, 10 (5), 555, https://doi.org/10.3390/antibiotics10050555
  6. Kafantaris, I., Tsadila, C., Nikolaidis, M., Tsavea, E., Dimitriou, T.G., Iliopoulos, I., Amoutzias, G.D., Mossialos, D. Transcriptomic analysis of pseudomonas aeruginosa response to pine honey via rna sequencing indicates multiple mechanisms of antibacterial activity (2021) Foods, 10 (5), 936, https://doi.org/10.3390/foods10050936
  7. Daskou, M., Tsakogiannis, D., Alexopoulou, D.S., Dimitriou, T.G., Mossialos, D., Amoutzias, G.D., Kottaridi, C., Markoulatos, P. A colorimetric IsoPCR for the rapid and sensitive visual detection of high-risk HPV16 in clinical samples with hydroxynaphthol blue (2021) Journal of Virological Methods, 290, 114072, https://doi.org/10.1016/j.jviromet.2021.114072
  8. Daskou, M., Dimitriou, T.G., Alexopoulou, D.S., Tsakogiannis, D., Amoutzias, G.D., Mossialos, D., Kyriakopoulou, Z., Markoulatos, P. WarmStart colorimetric RT-LAMP for the rapid, sensitive and specific detection of Enteroviruses A–D targeting the 5′UTR region (2021) Journal of Applied Microbiology, 130 (1), pp. 292-301 https://doi.org/10.1111/jam.14770
  9. Pleeging, C.C.F., Coenye, T., Mossialos, D., Rooster, H., Chrysostomou, D., Wagener, F.A.D.T.G., Cremers, N.A.J. Synergistic antimicrobial activity of supplemented medical-grade honey against pseudomonas aeruginosa biofilm formation and eradication (2020) Antibiotics, 9 (12), art. no. 866, pp. 1-16 https://doi.org/10.3390/antibiotics9120866
  10. Didaras, N.A., Karatasou, K., Dimitriou, T.G., Amoutzias, G.D., Mossialos, D. Antimicrobial activity of bee-collected pollen and beebread: State of the art and future perspectives (2020) Antibiotics, 9 (11), art. no. 811, pp. 1-29 https://doi.org/10.3390/antibiotics9110811
  11. Daskou, M., Dimitriou, T.G., Kouklamani-Giannouli, G., Nikolaidis, M., Mossialos, D., Amoutzias, G.D., Markoulatos, P. Development of a reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) that detects enteroviruses by targeting the highly conserved 5′-UTR region (2020) Virus Genes, 56 (2), pp. 194-201 https://doi.org/10.1007/s11262-020-01732-w
  12. Kafantaris, I., Amoutzias, G.D., Mossialos, D. Foodomics in bee product research: a systematic literature review (2021) European Food Research and Technology, 247 (2), pp. 309-331 https://doi.org/10.1007/s00217-020-03634-5
  13. Tsavea, E., Mossialos, D. Antibacterial activity of honeys produced in Mount Olympus area against nosocomial and foodborne pathogens is mainly attributed to hydrogen peroxide and proteinaceous compounds (2019) Journal of Apicultural Research, 58 (5), pp. 756-763 https://doi.org/10.1080/00218839.2019.1649570
  14. Daskou, M., Tsakogiannis, D., Dimitriou, T.G., Amoutzias, G.D., Mossialos, D., Kottaridi, C., Gartzonika, C., Markoulatos, P. WarmStart colorimetric LAMP for the specific and rapid detection of HPV16 and HPV18 DNA (2019) Journal of Virological Methods, 270, pp. 87-94 https://doi.org/10.1016/j.jviromet.2019.04.023
  15. Daskou, M., Tsakogiannis, D., Dimitriou, T.G., Manali, M., Apti, C., Amoutzias, G.D., Mossialos, D., Kottaridi, C., Markoulatos, P. Α 2-stage, nested-like nucleic acid amplification method (IsoPCR) for the highly sensitive detection of HPV16 and HPV18 DNA (2019) Molecular and Cellular Probes, 45, pp. 1-7 https://doi.org/10.1016/j.mcp.2019.03.003

Full publication list

PhD Thesis

  1. Asoutis-Didaras Nikolaos: Investigation of bioactivity & microbiome in Greek Beebread (in progress)
  2. Tsadila Christina: Characterization of biological activity of Microbiome and Antimicrobial mechanisms of Greek Honeys (in progress)
  3. Daskou Maria: Molecular monitoring of inactivation efficiency of Enteroviruses strains Sabin 1, Coxsackie A13 and Echovirus 12 by using an integrated: Cell culture/Real Time RT-PCR and real time RT-LAMP. Impact of inactivation on viral genome (2021)
  4. Dimitriou Tilemachos: Study of the mechanisms of in vitro preparation of recombinant viral strains between Sabin strains and strains of C Enteroviruses (2017)
  5. Tsakogiannis Dimitrios: Determination of the physical status of HPV 16 genome, correlation with the CINs profile of patients and determination of the integration site of HPV16 in human genome (2014)
  6. Ruether Irene: Molecular and phylogenetic analysis of Noroviruses (2014)
  7. Kyriakopoulou Zaharoula: Molecular detection of enteroviruses in the environment and characterization of recombinations sites in circulating enteroviruses in the environment (2010)
  8. Pliaka Vaia: Molecular detection of Noroviruses-Polioviruses in the environment. Identification of genetic recombinations and correlation with the replication efficiency of viral strains (2010)
  9. Paximadi Eleni: Recombinant poliovirus strains – sites of genetic recombination (2009)
  10. Dedepsidis Evaggelos: Poliovirus OPV strains- Correlation between recombination and RNA secondary structures (2008)
  11. Kottaridi Christina: Molecular identification of genome evolution of Echoviruses (2007)

Laboratory of Microbial Biotechnology – Molecular Bacteriology – Virology

Department of Biochemistry and Biotechnology (1st Floor)
University of Thessaly
Viopolis, 41500 – Larissa