Content – Aim of the course
The Molecular Biology course is tailored for students of the Department of Biochemistry and Biotechnology who have already acquired basic knowledge of “General” and “Organic Chemistry”, “Structure and Analysis of Biomolecules” and “Biosynthesis of macromolecules – regulation of gene expression”. This course takes a deeper look into issues such as DNA damage and repair, recombination, transposition and regulation. The aim is, as far as possible, to do this not so by the sheer presentation of the relevant information, as through the understanding of the experimental proof that led to the acquisition of that information.
The presentations are grouped into two cycles. The first cycle (Lessons 1-5) starts with a display of selected laboratory techniques such as cloning, library construction, hybridization, PCR and site-directed mutagenesis. Then follows an analysis of various types of DNA damage and the mechanisms that cells use to repair them, including homologous recombination. This leads to the discussion of transposition of mobile elements in prokaryotes and eukaryotes. The second cycle (Lessons 6-11) studies the regulation of gene transcription in both prokaryotes and eukaryotes. During these lectures there is a detailed analysis of the structure and function of bacterial and eukaryotic RNA polymerases, mRNA synthesis and the regulatory mechanisms of gene expression. Finally, how epigenetic modifications affect gene expression is also considered.