Analytical Biochemistry
Theory: 2 hours/week | Tutorials: 2 hours/week | ECTS Units: 5
Content – Aim of the course
Recent advances in basic science and especially in biochemistry and molecular biology were based in the development of new methodologies and techniques. This course introduces experimental techniques that are broadly used in every biochemical laboratory. The primary goal is the understanding of the theoretical background of many methods that are applied in biochemistry and molecular biology and the design and execution of experimental protocols. In addition, based on oral presentations, the students are introduced to techniques like DNA arrays, Flow cytometry analysis, Proteomic analysis, Bioimaging and bio-molecular interaction analysis.
Analytical Description of the Course
- BASIC TECHNIQUES IN EXPERIMENTAL BIOCHEMISTRY.
- GEL ELECTROPHORESIS.
- ULTRA CENTRIFUGATION.
- DNA – RNA ISOLATION AND QUANTIFICATION.
- REAL-TIME PCR.
- LASERS TO STUDY THE DYNAMICS OF BIOLOGICAL SYSTEMS (FACS analysis. Confocal Microscopy).
- PROTEIN ISOLATION AND CHARACTERIZATION.
- HPLC – FPLC.
- X-RAY CRYSTALLOGRAPHY OF PROTEINS.
- MICROARRAYS.
- PROTEOMICS.
- BIOMOLECULAR INTERACTION ANALYSIS.
Assessment
Students are assessed after written exams at the end of the course.
Reading Suggestions
- Experimental Biochemistry, Clark Jr. J.M. and Switzer R.L., Crete University Press, 1992.
- Analytical Biochemistry, Holme D. and Peck H., Pearson Education eds, 2001.
- Genome Analysis: A laboratory manual series (vol. 1, 2, 3, 4), Green Ε.D., Birren B., Klapholz S., Myers R.M., Hieter P., CSHL Press, 1997.
Teaching Material / E-class
Lecturer
